Method for lysing Mycobacteria using achromopeptidase
US5185242A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jun 24, 1991 |
| Grant date | Feb 9, 1993 |
| Priority date | — |
| Expiry date | Jun 24, 2011 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/35
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention provides a rapid method for lysing Mycobacteria. In one embodiment is provided a method for lysing Mycobacteria which comprises exposing the bacteria to a lysis effective amount of the enzyme achromopeptidase. The method of the invention is particularly advantageous since only one step is involved, it is expedient compared to prior methods, and little instrumentation is necessary. By practicing the present invention it is possible to lyse Mycobacteria with minimal effort. In addition, practicing the invention results in liberating cellular components including deoxyribonucleic acid (DNA) from Mycobacteria. Not only is DNA liberated, but the DNA is suited for subsequent analysis by way of probe hybridization, restriction enzyme analysis, and the like.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.