Detection of influenza a virus by polymerase chain reaction (PCR) preceded by reverse transcription of a region of the viral hemagglutinin gene
US5187060A · kind A · utility
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8Claims
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Key dates
| Filing date | Aug 31, 1990 |
| Grant date | Feb 16, 1993 |
| Priority date | — |
| Expiry date | Aug 31, 2010 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/701
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
It was possible to increase substantially the sensitivity of detection of influenza A virus by carrying out a polymerase chain reaction (PCR), using oligonucleotide sequences (amplimers) from the relatively conserved region of the viral hemagglutinin gene, preceded by reverse transcription (RT). The reverse transcription and the PCR are insensitive to single mutations by base exchange in this case, as long as the 3' end of the amplimer is not affected.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.