Rapid purification of DNA
US5187083A · kind A · utility
91Cited by
3References
29Claims
0Family size
Assignee
Inventor
Key dates
| Filing date | Nov 13, 1990 |
| Grant date | Feb 16, 1993 |
| Priority date | — |
| Expiry date | Nov 13, 2010 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/82
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides a method for rapidly obtaining substantially pure DNA from a biological sample containing cells. The method involves gently lysing the membranes of the cells to yield a lysate containing genomic DNA in a high molecular weight form. The lysate is moved through a porous filter to selectively trap the high molecular weight DNA on the filter. The DNA is released from the filter using an aqueous solution to form a solution containing substantially purified DNA, from which the DNA is recovered.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.