Method and apparatus for homogeneous fluorescence measurements
US5187106A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Aug 30, 1984 |
| Grant date | Feb 16, 1993 |
| Priority date | — |
| Expiry date | Aug 30, 2004 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S436/80
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A fluorescence decay time method and apparatus directly measuring the time decay function resulting from a plurality of fluorescing bodies, including background, and analytically determines the presence and concentration of the fluorescing bodies homogeneously and simultaneously is disclosed. The fluorescently labelled reaction product of a target ligand, such as an antigen, with a binding molecule for the ligand, such as an antibody, exhibits a fluorescent decay time curve which bears a direct straight line relationship to the concentration of the target antigen, in that the value for fluorescent intensity at time zero for the decay time curve is a measure of said concentration. The time zero intensity is proportional to the concentration of the target ligand in the unknown sample which can then be compared to the concentrations from a standard curve.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.