Enzymatic synthesis of soluble phosphatides from phospholipids
US5188951A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | May 1, 1991 |
| Grant date | Feb 23, 1993 |
| Priority date | — |
| Expiry date | May 1, 2011 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12P13/00
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
A method of preparing a soluble monovalent salt of a phosphatidyl ester which comprises reacting a phospholipid with a primary alcohol in the presence of an enzyme catalyst in a divalent cationic buffered solution and a water immiscible non-ether solvent that does not inactivate the enzyme, to form a divalent cationic salt of the phosphatidyl ester, and suspending the product in the presence of a stoichiometric amount of a monovalent cationic salt whose anion forms an insoluble salt with the divalent cation. The use of Centrifugal Partition Chromatography facilitates the enzyme reaction. The monovalent salt is preferably an ammonium/sodium mixed salt.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.