Biosensor utilizing enzyme and a method for producing the same
US5192415A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Mar 2, 1992 |
| Grant date | Mar 9, 1993 |
| Priority date | — |
| Expiry date | Mar 2, 2012 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/004
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The biosensor of the present invention quantifies a substrate contained in a sample liquid by reducing electron acceptors using electron generated in a reaction of the substrate to enzyme and then by measuring the reduced amount of the electron acceptors electrochemically. The biosensor has an electrical insulating substrate, an electrode system including at least a working electrode and a counter electrode, a reaction layer including the enzyme provided on the electrode system and a hydrogen ion concentration control layer, and the reaction layer is in contact with the electrode system. According to the present invention, the hydrogen ion concentration in the sample liquid can be made most appropriate in accordance with the type of the enzyme contained in the reaction layer, without the adjustment of the hydrogen ion concentration in the sample liquid beforehand. Thus, the specific substrate contained in the sample liquid can be easily quantified with accuracy and speed.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.