Method to produce recombinant proteins using an expression vector comprising transcriptional and translational activating sequences
US5192669A · kind A · utility
13Cited by
2References
28Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Aug 13, 1991 |
| Grant date | Mar 9, 1993 |
| Priority date | — |
| Expiry date | Aug 13, 2011 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/70
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention is composed of novel recombinant DNA expression vectors which contain a transcriptional activating sequence, a translational activating sequence and a DNA sequence coding for a functional polypeptide, especially bovine growth hormone. The aforementioned translational activating sequences contain a ribosome binding site and are designed to provide high level expression of DNA that codes for virtually any functional polypeptide. The invention further provides transformed microbial host cells capable of producing bovine growth hormone and other functional polypeptides at high levels.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.