Cephalosporin C acylase
US5192678A · kind A · utility
17Cited by
0References
2Claims
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Assignee
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Key dates
| Filing date | Aug 20, 1991 |
| Grant date | Mar 9, 1993 |
| Priority date | — |
| Expiry date | Aug 20, 2011 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12P35/04
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A cephalosporin C acylase from Pseudomonas diminuta N-176 is disclosed as well as the recombinant production of this enzyme in E. coli. The enzyme is characterized by the ability to catalyze the conversion of cephalosporin C, gultaryl 7-ACA, apidyl 7-ACA, succinyl 7-ACA, N-acetylcephalosporin C, N-benzoylcephalosporin C, and cephalothin into 7-aminocephalosporanic acid and is composed of an .alpha.-subunit with a molecular weight of 26,000 daltons and a .beta.-subunit with a molecular weight of 58,000 daltons.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.