Method of determining endothelial cell coverage of a prosthetic surface
US5194373A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Nov 20, 1990 |
| Grant date | Mar 16, 1993 |
| Priority date | — |
| Expiry date | Nov 20, 2010 |
Classification
- Technology area (CPC A)Human Necessities
- CPC primaryA61F2/062
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Determination of effectiveness of microvascular endothelial cell seeding upon a vascular graft surface within the operating room environment would be desirable to maintain quality control in any clinical trial. A number of fluorescent dyes including mithramycin, Hoechst 33342, sulfofluorescein diacetate, Nile Red, rhodamine 123, and PKH26-GL were evaluated for their ability to fluorescently label uncultured microvascular endothilial cells on graft material and subsequently allow determination of seeded cell number and cell spreading. Rhodamine 123 and PKH26-GL produced the most desirable characteristics. The selected non-toxic fluorescent dyes allowed excellent cell visualization after a 30 minute incubation. Unlike the other fluorescent dyes evaluated, the selected non-toxic fluorescent dyes caused the cellular cytoplasm to fluoresce bright orange at a 510 nm excitation wavelength while the underlying polyethyleneterephthalate polyester or expanded polytetrafluorethylene demonstrated minimal autofluorescence. No inhibitory effect on cell attachment to plastic or subsequent cell growth in culture was observed. This technique is useful in the operating room to visualize part or all …
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.