Apparatus and method for measuring volume and hemoglobin concentration of red blood cells
US5194909A · kind A · utility
Inventor
Key dates
| Filing date | Dec 4, 1990 |
| Grant date | Mar 16, 1993 |
| Priority date | — |
| Expiry date | Dec 4, 2010 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/808
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
An apparatus and method for measuring the volume and hemoglobin concentration of individual red blood cells in a whole blood sample provides for isovolumetrically sphering the red blood cells of the sample using a sphering reagent and forming a stream of individual, spaced-apart cells sheathed in an electrolytic liquid in a flow chamber. The red blood cells in the stream are passed through a light scattering channel in which the intensity of light scattered by each cell into a preselected angular interval in the forward direction is measured. Each cell in the stream is also passed through a resistance pulse sizing aperture for measuring the volume of the cell. The angular interval within which the intensity of scattered light intensity is measured is selected such that for each measured value of scattered light intensity corresponding to a red blood cell and value of the volume of the cell, as determined by the resistance pulse sizing, a unique value of hemoglobin concentration for the cell may be calculated over the entire physiological range of values of cell volume and cell hemoglobin concentration. The values of the cell volume and cell hemoglobin concentration are then used fo…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.