Diagnostic and amplification methods using primers having thymine at 3' end to overcome primer-target mismatch at the 3' end
US5196305A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Sep 12, 1989 |
| Grant date | Mar 23, 1993 |
| Priority date | — |
| Expiry date | Sep 12, 2009 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S436/811
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods for amplifying and detecting a predetermined target nucleic acid in a biological specimen are accomplished even where there is a mismatch in a single position between a primer and the target nucleic acid. The mismatch is located at or near the 3' end of the primer. Such a mismatch is overcome using a primer having a nucleotide with a thymine base at the position of the mismatch. The use of such primers is most likely to prime the target and form primer extension products. This method is particularly useful for detection of a nucleic acid sequence which is not fully known, or where there is considerable heterogeneity in DNA target from patient samples.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.