Patent · US Expired

Overexpression of proteins in recombinant host cells

US5196317A · kind A · utility

2Cited by

1References

14Claims

0Family size

Assignee

Merck Patent GmbH · DE

Inventors

Martin Heinrich · Cedar Grove, US
Wolfgang Ebeling · Hannover, DE
Wolfgang Brummer

Key dates

Filing date	Nov 2, 1990
Grant date	Mar 23, 1993
Priority date	—
Expiry date	Nov 2, 2010

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12N15/73
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

The invention relates to a process for achieving high, thermally-induced expression rates, which last for a relatively long period of time, of proteins in recombinant cells, preferably in E. coli cells, by optimizing cell density, induction temperature and supplemented amino-acid-containing substrates.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.