Generation and selection of novel DNA-binding proteins and polypeptides
US5198346A · kind A · utility
254Cited by
17References
48Claims
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Key dates
| Filing date | Jul 26, 1990 |
| Grant date | Mar 30, 1993 |
| Priority date | — |
| Expiry date | Jul 26, 2010 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2795/10322
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
Novel DNA-binding proteins, especially repressors of gene expression, are obtained by variegation of genes encoding known binding proteins and selection for proteins binding the desired target DNA sequence. A novel selection vector may be used to reduce artifacts. Heterooligomeric proteins which bind to a target DNA sequence which need not be palindromic are obtained by a variety of methods, e.g., variegation to obtain proteins binding symmetrized forms of the half-targets and heterodimerization to obtain a protein binding the entire asymmetric target.
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