Preparation of a blood platelet lysate for use in a cell culture medium for hybridoma cells
US5198357A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Dec 26, 1990 |
| Grant date | Mar 30, 1993 |
| Priority date | — |
| Expiry date | Dec 26, 2010 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2500/90
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A platelet lysate is produced from plasma derived from animal whole blood that contains an added citrate to prevent coagulation of the blood during storage. The lysate is prepared by centrifuging plasma to produce a platelet rich paste, adding calcium to the paste to lyse the platelets therein and coagulate fibrinogen to produce a clear liquid containing lysed platelets, sterile filtering the liquid and collecting a liquid filtrate containing the lysed platelets. The obtained lysate of platelets can be used to wholly or partly replace foetal calf serum in cell culture, such as in culture of hybridoma cells for preparing monoclonal antibodies.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.