Digoxigenin derivatives and use thereof

US5198537A · kind A · utility

Assignee

• Boehringer Mannheim GmbH · DE

Inventors

• Erasmus Huber · Garching bei München, DE
• Klaus Muhlegger · Polling, DE
• Herbert von der Eltz · Spaichingen, DE
• Bruno Zink · Murnau am Staffelsee, DE

Key dates

• Filing date: Oct 25, 1989
• Grant date: Mar 30, 1993
• Expiry date: Oct 25, 2009

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N33/53
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The present invention provides digoxigenin derivatives of the formula: ##STR1## wherein n is a whole number of from 1 to 4 and Z is a carboxyl group or a functional derivative derived therefrom. The present invention also provides digoxigenin conjugates of the general formula: ##STR2## wherein the carrier is an immunogenic carrier material, a nucleic acid or a labelled immunocomponent of a labelled digoxigenin immunoconjugate for use in an immunoassay, m is a number from 1 to the number of coupling positions available on the carrier and Y is the group formed by the reaction of the carboxyl function Z of the digoxigenin derivative of general formula (I) with the reacting position of the carrier material. Also described are uses of these conjugates as nucleic acid probes, immunogens, and in immunoassay.