Preparation of gel-filled separation columns
US5200150A · kind A · utility
11Cited by
5References
9Claims
0Family size
Assignee
Inventor
Key dates
| Filing date | Jun 28, 1991 |
| Grant date | Apr 6, 1993 |
| Priority date | — |
| Expiry date | Jun 28, 2011 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N27/44704
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method of preparing a microcapillary gel separation column for electrophoresis comprises: pretreatment of a tube with a bifunctional reagent to provide polymer bonding sites on the inner wall of the tube; filling the tube with a monomer solution a cross-linking agent, and a photoinitiator; forming a polymer plug by illuminating an initiation section of the tube; growing the plug by moving the tube gradually past the illumination source; and stopping tube motion when the desired length of gal is attained. Provision is made for monitoring polymerization and adjusting tube movement speed to avoid gaps in the growing polymer.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.