Nucleic acid hybridization assay employing detectable anti-hybrid antibodies
US5200313A · kind A · utility
126Cited by
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9Claims
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Key dates
| Filing date | Apr 25, 1988 |
| Grant date | Apr 6, 1993 |
| Priority date | — |
| Expiry date | Apr 25, 2008 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S436/808
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A nucleic acid hybridization assay employing an immobilized or immobilizable polynucleotide probe selected to form DNA.RNA or RNA.RNA hybrids with the particular polynucleotide sequence to be determined. Resulting hybrids are detected by binding of an antibody reagent, preferably labeled with a detectable chemical group, selective for binding the hybrids in the presence of the single stranded sample and probe nucleic acids. No immobilization or labeling of sample nucleic acids is necessary and hybridization can be performed entirely in solution.
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