Process for the detection of substances with hydrolase activity

US5202233A · kind A · utility

Assignee

• Boehringer Mannheim GmbH · DE

Inventors

• Rupert Herrmann · Weilheim, DE
• Hans-Joachim Guder · Weilheim, DE
• Werner Guthlein · Mannheim, DE
• Manfred Kuhr
• Johann Berger · Alfdorf, DE
• Harvey Buck · Indianapolis, US

Key dates

• Filing date: Jan 3, 1992
• Grant date: Apr 13, 1993
• Expiry date: Jan 3, 2012

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S435/805
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

A process for the detection of substances with hydrolase activity in a sample by mixing the sample with a hydrolase substrate and an oxidizing agent, and the evaluating the resultant color intensity, wherein, as hydrolase substrate, there is used at least one compound of the formula ##STR1## in which R.sup.1 is hydrogen or an alkoxy radical, PA0 R.sup.2 is hydrogen or halogen, an amino group or an alkoxy or aralkoxy radical, PA0 R.sup.3, R.sup.4, R.sup.5 and R.sup.6, which can be the same or different, are hydrogen, halogen, carboxyl, a carbamoyl group, sulpho, an amino groups alkyl, an alkoxy radical, aralkoxy, alkylcarbonyl or alkoxycarbonyl, and PA0 X is a glycosyl, phosphate or acyl residue. Optionally R.sup.2 and R.sup.3, R.sup.3 and R.sup.4, R.sup.4 and R.sup.5, and R.sup.5 and R.sup.6 can be joined to form a ring system. At least one of R.sup.1 and R.sup.2 must be hydrogen and at least one of R.sup.1, R.sup.2 and R.sup.3 must not be hydrogen. Compounds in which X is .beta.-galactosyl are new.