Expression of recombinant polypeptides with improved purification
US5202239A · kind A · utility
22Cited by
7References
8Claims
0Family size
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Key dates
| Filing date | Aug 7, 1990 |
| Grant date | Apr 13, 1993 |
| Priority date | — |
| Expiry date | Aug 7, 2010 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2319/75
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
An improved method for expressing peptides as fusion proteins, uses a carrier for a heterologous peptide to provide a fusion protein having a high pI. The high isoelectric point facilitates separation of the fusion protein from all other host cell proteins, and separation of the carrier from the peptide after cleavage.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.