Patent · US Expired

Process for the reduction of star activities

US5204238A · kind A · utility

3Cited by

0References

9Claims

0Family size

Assignee

Boehringer Mannheim GmbH · DE

Inventors

Frank Laue · Kempten (Allgäu), DE
Waltraud Ankenbauer · Penzberg, DE
Gudrun Schmitz · Deggendorf, DE

Key dates

Filing date	Mar 14, 1991
Grant date	Apr 20, 1993
Priority date	—
Expiry date	Mar 14, 2011

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12P19/34
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

The present invention provides a process for the reduction of non-specific star activities in the case of the specific cleavage of desoxyribonucleic acids by incubation with a restriction endonuclease in an appropriate buffer, wherein to the incubation batch is added an antibiotic which binds to the DNA on or near the star sequences of the enzyme but not within the specific recognition sequence of the restriction endonuclease.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.