Method test media and chromogenic compounds for identifying and differentiating general coliforms and Escherichia coli bacteria
US5210022A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Apr 20, 1990 |
| Grant date | May 11, 1993 |
| Priority date | — |
| Expiry date | Apr 20, 2010 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/924
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A chromogenic .beta.-galactosidase substrate producing an insoluble precipitate of a first color when reacted upon by .beta.-galactosidase and a chromogenic .beta.-glucuronidase substrate producing an insoluble precipitate of a second, contrasting color when reacted upon by .beta.-glucuronidase are combined in a test medium for quantitatively identifying and differentiating general coliforms and E. coli. The .beta.-galactosidase substrate 5-bromo-4-chloro-3-indolyl-.beta.-D-galactopyranoside which produces an indigo blue precipitate when reacted upon by .beta.-galactosidase may be used with one of the novel compounds 6-chloroindolyl-.beta.-D-glucuronide, 4,6-dichloroindolyl-.beta.-D-glucuronide, 6,7-dichloroindolyl-.beta.-D-glucuronide, and 4,6,7-trichloroindolyl-.beta.-D-glucuronide, which produce mauve or magenta precipitates when reacted upon by .beta.-glucuronidase. The .beta.-glucuronidase substrate 5-bromo-4-chloro-3-indolyl-.beta.-D-glucuronide, which produces an indigo blue precipitate when reacted upon by .beta.-glucuronidase may be used with one of the novel compounds 6-chloroindolyl-.beta.-D-galactoside, 4,6-dichloroindolyl-.beta.-D-galactoside, 6,7-dichloroindolyl-.beta…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.