Secretory signal selection vectors for extracellular protein synthesis in Bacilli
US5212070A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Dec 13, 1990 |
| Grant date | May 18, 1993 |
| Priority date | — |
| Expiry date | Dec 13, 2010 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2319/61
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Novel vectors are provided for identifying secretory signal sequences from DNA fragments of unicellular microorganisms. The plasmids comprise a multiple cloning site with restriction sites in reading frame with a structural gene which permits rapid screening of the secreted expression product. Optionally, the vectors may include a promoter region upstream from the multiple cloning site. The invention is exemplified with Bacillus. Specific secretory signal sequences have been isolated with those vectors, allowing for efficient secretion into the supernatant, and not just to the periplasmic space to provide proteins in economically high yields. Secretory sequences are provided superior to other previously known sequences.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.