Quantitative determination of glucose utilizing enzyme cascade system
US5213966A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Sep 25, 1989 |
| Grant date | May 25, 1993 |
| Priority date | — |
| Expiry date | Sep 25, 2009 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/144444
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A procedure is disclosed for the determination of glucose in a biological liquid, as well as a reagent mixture for use in conjunction with the procedure. The biological liquids concerned are especially blood, blood components, urine and spinal fluid. The procedure is based on a two-phase enzymatic reaction, the first phase of which comprises transforming glucose with the aid of ATP (adenosine-5-triphosphate) and hexokinase into glucose-6-phosphate and the second phase of which comprises transforming glucose-6-phosphate with the aid of NAD and glucose-6-phosphate dehydrogenase into 6-phosphogluconate. ATP, NAD and the enzymes mentioned are included in a suitably buffered reagent mixture, which can be added as a single dose to the glucose-containing liquid sample. The reaction is monitored kinetically by measuring the absorption caused by the NADH produced in the second phase twice within approximately one minute from the initiation of the first reaction phase. The glucose content of the sample can be calculated from the measured change of absorbance.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.