Method of immobilizing nucleic acid on a solid surface for use in nucleic acid hybridization assays
US5215882A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Nov 30, 1989 |
| Grant date | Jun 1, 1993 |
| Priority date | — |
| Expiry date | Nov 30, 2009 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S436/808
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Methods of immobilizing nucleic acid on a solid surface for us in nucleic acid hybridization assays is disclosed. The methods of the invention comprise reacting a modified nucleic acid strand comprising a variable portion and an anchor portion wherein the variable portion comprises a nucleotide sequence having a selected base sequence and the anchor portion comprises at least one nucleotide base modified with a primary amine function or nucleotide base equivalent having a primary amine function and reacting the modified nucleic acid strand with a free aldehyde group of the solid surface in the presence of a reducing agent to form complexes of the modified nucleic acid strand and at least a portion of the free aldehyde groups on the solid surface.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.