Electrophoretic mobility of fluorophore labeled particles in gels by fluorophore movement after photobleaching
US5215883A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Jul 9, 1990 |
| Grant date | Jun 1, 1993 |
| Priority date | — |
| Expiry date | Jul 9, 2010 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N27/44721
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A process and apparatus for detecting the mobility of fluorescently labeled molecules in response to a force is disclosed. The fluorescently labeled molecules are placed in a fluid medium such as an electrophoretic gel in a capillary tube. An electric field is applied to induce the movement of the molecules. A region of the labeled molecules is photobleached leaving a geometrically defined region which has not been photobleached. The region which has not been photobleached is excited by a reading beam. The reading and the photobleaching beams can be generated by focusing a laser through a diffraction grading. The intensity produced by the interaction of the reading beam in the geometrically defined region of the molecule is detected by a photo detector. In detecting more than one sp This invention was partially made with Government support under the polymers program of the National Science Foundation DMR8617820. The Government has certain rights in the invention.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.