Polynucleotide assay reagent and method
US5217866A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Dec 18, 1986 |
| Grant date | Jun 8, 1993 |
| Priority date | — |
| Expiry date | Dec 18, 2006 |
Classification
- Technology area (CPC B)Performing Operations; Transporting
- CPC primaryB01J2219/0072
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
A diagnostic reagent and system for determination of a single-stranded polynucleotide analyte having a selected target sequence. The reagent includes a solid support and multiple support-bound polymers designed to bind specifically to the analyte. Each polymer is composed of a sequence of base-complementary recognition moieties which can bind specifically to corresponding contiguous bases in the analyte target sequence, and an unbranched, substantially uncharged, substantially stereoregular backbone. A reporter in the system contains (a) a polycationic tail effective to bind electrostatically to the analyte, under conditions in which the reporter does not bind to the substantially uncharged polymers, and (b) reporter groups by which the presence of the reporter can be detected.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.