Molecular cloning and expression of genes encoding proteolytic enzymes
US5217878A · kind A · utility
44Cited by
1References
18Claims
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Key dates
| Filing date | Feb 29, 1988 |
| Grant date | Jun 8, 1993 |
| Priority date | — |
| Expiry date | Feb 29, 2008 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/87
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Novel methods and novel industrial Bacillus strains are provided for enhanced production of serine protease. Plasmid constructs comprising the serine protease gene are introduced into a compatible host, generally a Bacillus already overproducing serine protease. Preferred host cells are those from Bacillus novo sp. PB92 and the preferred serine protease gene also originates from Bacillus PB92. Integration and maintenance of the plasmid in the host cell chromosome can be achieved by including a temperature sensitive origin of replication in the plasmid construct and growing under selective conditions.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.