Template-directed photoligation

US5219734A · kind A · utility

Assignee

• Amoco Corporation · US

Inventors

• Garfield P. Royer · Upperville, US
• Larry E. Morrison · Glen Ellyn, US
• Kenneth A. Cruickshank · Naperville, US

Key dates

• Filing date: Jan 13, 1988
• Grant date: Jun 15, 1993
• Expiry date: Jan 13, 2008

Classification

• Technology area (CPC —): General

Abstract

Methods, apparatus and compositions are presented for ligating ligands together which bind to a common target. One embodiment includes polynucleotide probes having photoreactive functional groups. The probes are capable of assuming substantially contiguous reactive positions on a target polynucleotide placing the photoreactive groups in juxtaposition. Activation of the photoreactive functional groups with radiant energy form a probe reaction product in which the probes are bound to each other.