Mutant luciferase of a firefly, mutant luciferase genes, recombinant DNAS containing the genes and a method of producing mutant luciferase
US5219737A · kind A · utility
40Cited by
2References
15Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Mar 26, 1991 |
| Grant date | Jun 15, 1993 |
| Priority date | — |
| Expiry date | Mar 26, 2011 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y113/12007
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides industrially useful luciferase. Mutant luciferase of the invention is produced by culturing a microorganism belonging to the genus Escherichia which harbors a recombinant DNA containing the mutant luciferase gene of a firefly. Mutant luciferase can produce red, orange or green color of light which can not be produced by wild type luciferase. Mutant luciferase can be used to measure ATP accurately in a colored solution such as red (e.g., blood), orange, or green in which wild-type luciferase has not provided reliable results.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.