Agglutination method for the determination of multiple ligands
US5219763A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Aug 3, 1990 |
| Grant date | Jun 15, 1993 |
| Priority date | — |
| Expiry date | Aug 3, 2010 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S436/805
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The invention provides a method capable of determining the presence or absence of each of a plurality of different ligands in a specimen. The specimen is contacted with a predetermined number of different homogenous populations of fluorescent beads having one or more predetermined antiligands affixed to their surface. The specimen and bead mixture is analyzed using a means having a single parameter of measuring fluorescence per ligand to determine the number of non-agglutinated beads, the number of agglutinated beads, the number of bead aggregates, and for each aggregate, the number of beads its comprises. This information is used to correlate the presence or absence in the specimen of each of the different ligands analyzed for. The method of the invention thus provides for the simultaneous determination of a predetermined number of ligands in a specimen using only a single bead contacting step.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.