Methods for rendering amplified nucleic acid subsequently unamplifiable

US5221608A · kind A · utility

Inventors

• George D. Cimino · Richmond, US
• John E. Hearst · Berkeley, US
• Stephen T. Isaacs · Orinda, US
• Kenneth C. Metchette · Berkeley, US
• John W. Tessman · Berkeley, US

Key dates

• Filing date: Oct 26, 1989
• Grant date: Jun 22, 1993
• Expiry date: Oct 26, 2009

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S436/805
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Method for treating nucleic acid that is to be amplified such that the nucleic acid is, after amplification, subsequently unamplifiable. Nucleic acid amplification is performed in a reaction containing means in the presence of an isopsoralen. The reaction is thereafter irradiated to render the amplified nucleic acid substantially unamplifiable.