Cloning and expression of transforming growth factor .beta.2
US5221620A · kind A · utility
41Cited by
5References
2Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Dec 5, 1989 |
| Grant date | Jun 22, 1993 |
| Priority date | — |
| Expiry date | Dec 5, 2009 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2319/00
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
cDNA clones coding for TGF-.beta.2 which are used to construct expression vectors capable of directing the high-level expression of mature, biologically active TGF-.beta.2, as well as precursor TGF-.beta.2 forms, in transfected Chinese Hamster Ovary cells (CHO cells) and transfected COS cells are described. CHO and COS transfectants secreting TGF-.beta.2 at high levels are also described. CHO cells transfected with a plasmid vector carrying the complete 414 amino acid simian TGF-.beta.2 precursor secrete approximately 5 .mu.g per ml culture media.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.