Detection of analytes having binding sites for at least two binding moieties
US5223393A · kind A · utility
22Cited by
9References
19Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Jun 12, 1990 |
| Grant date | Jun 29, 1993 |
| Priority date | — |
| Expiry date | Jun 12, 2010 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/581
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention provides reagents for a detectable label for use in specific binding assays. Specifically, modified .beta.-galactosidase enzyme donors (ED) and acceptors (EA) are utilized. Both ED and EA are modified to form separate ED and EA complexes by coupling each with a linking element and a binding moiety which is specific to a binding site in the analyte. The ED and EA complexes are incapable of forming active enzyme in the absence of the analyte. However, when analyte is present, ED and EA complexes which bind to a common analyte in a sample, active .beta.-galactosidase is formed.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.