Immunometric assays for tumor necrosis factor-alpha and methods for preventing the loss of biological activity of tumor necrosis factor-alpha in biological samples
US5223395A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Dec 1, 1988 |
| Grant date | Jun 29, 1993 |
| Priority date | — |
| Expiry date | Dec 1, 2008 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/6863
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
An immunometric assay is disclosed for biologically active tumor necrosis factor in a biological sample comprising, forming a complex of a first labeled monoclonal antibody, biologically active TNF, and a second monoclonal antibody which can be bound to an insoluble substrate and detecting the amount of label associated with the complex. The assay is characterized by employing first and second monoclonal antibodies which react with the same epitopic site on TNF-alpha monomer. A method is also disclosed for blocking accelerated TNF degradation in a non-preserved biological sample, such as blood, comprising contacting the biological sample with EDTA, luminol, or a combination thereof.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.