Patent · US Expired

Cell-free and whole cell ice nucleators and process for their production

US5223412A · kind A · utility

7Cited by
10References
26Claims
0Family size

Assignee

Inventors

Key dates

Filing dateFeb 21, 1991
Grant dateJun 29, 1993
Priority date
Expiry dateFeb 21, 2011

Classification

  • Technology area (CPC Y)Emerging Cross-Sectional Technologies
  • CPC primaryY10S530/828
  • WIPO fieldBiotechnology
  • WIPO sectorChemistry

Abstract

Microbially produced ice nucleator mixtures which include either cell-free ice nucleator particle mixtures and/or whole cell ice nucleator mixtures. These mixtures are produced in methods which comprises culturing a selected microorganism in a two step process at a first temperature in a first step and at a lower temperature in a second step. The mciroorganisms include Erwinia, Pseudomonas and Escherichia coil. These methods produce ice nucleator mixtures having increased concentrations of ice nucleating sites per given weight or volume of ice nucleator material.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.