Cell-free and whole cell ice nucleators and process for their production
US5223412A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Feb 21, 1991 |
| Grant date | Jun 29, 1993 |
| Priority date | — |
| Expiry date | Feb 21, 2011 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S530/828
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Microbially produced ice nucleator mixtures which include either cell-free ice nucleator particle mixtures and/or whole cell ice nucleator mixtures. These mixtures are produced in methods which comprises culturing a selected microorganism in a two step process at a first temperature in a first step and at a lower temperature in a second step. The mciroorganisms include Erwinia, Pseudomonas and Escherichia coil. These methods produce ice nucleator mixtures having increased concentrations of ice nucleating sites per given weight or volume of ice nucleator material.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.