Process for the preparation of vancomycin
US5223413A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Sep 26, 1991 |
| Grant date | Jun 29, 1993 |
| Priority date | — |
| Expiry date | Sep 26, 2011 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/867
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention relates to a process for preparation of the vancomycin antibiotic in a microbiological way by aerobic fermentation, by using a strain belonging to the Micropolyspora species in a culture-medium containing assimilable carbon and nitrogen sources as well as mineral salts. According to the invention as microorganisms the aminoglycoside-resistant NCAIM 001092 and NCAIM 001093 mutants of the strain belonging to the Micropolyspora orientalis species are used. Nitrates are suitable mineral salts in the fermentation. Soy flour, glycerol, calcium gluconate, starch are useful carbon sources. The fermentation is carried out preferably at a temperature of 29.degree.-30.degree. C. The vancomycin antibiotic produced in the fermentation is separated by using reverse osmosis and ultrafiltration following ion-exchange chromatography.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.