Process for nucleic acid hybridization and amplification
US5223414A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | May 7, 1990 |
| Grant date | Jun 29, 1993 |
| Priority date | — |
| Expiry date | May 7, 2010 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6844
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention is directed to a method of achieving RecA protein facilitated amplification of a double-stranded DNA target sequence having first and second complementary strands, each strand with 5' and 3' ends. The method involves complexing a primer complementary to a 5' end region of the first strand and a primer complementary to a 5' end region of the second strand with RecA protein in the presence of ATP-.gamma.-S. The complexed primers are then reacted in a mixture also containing the target sequence, all four dNTPs, RecA protein and DNA polymerase. The reaction is conducted below the temperature required for thermal dissociation of the two target strands and continued until a desired degree of amplification of the target sequence is achieved. The present invention further includes the cloning and identification of the coding sequences for the RecA protein of Thermus aquaticus.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.