Method of improving the yield of heterologous proteins produced by Streptomyces lividans
US5223418A · kind A · utility
3Cited by
4References
6Claims
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Key dates
| Filing date | Sep 28, 1990 |
| Grant date | Jun 29, 1993 |
| Priority date | — |
| Expiry date | Sep 28, 2010 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/76
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method is provided for improving the yield of the heterologous proteins sCD4 and derivatives of sCD4, produced by recombinant Streptomyces lividans by the addition of casamino acids to the nutrient medium in which the bacteria are cultured. Also provided by this invention is a method for improving the half-life of heterologous proteins in a cell free culture supernatant by the addition of casamino acids to the supernatant. Complex media are also provided.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.