Peroxidase colloidal gold oxidase biosensors for mediatorless glucose determination
US5225064A · kind A · utility
64Cited by
5References
15Claims
0Family size
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Key dates
| Filing date | Mar 6, 1992 |
| Grant date | Jul 6, 1993 |
| Priority date | — |
| Expiry date | Mar 6, 2012 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/817
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Horseradish peroxidase (HRP) immobilized on colloidal gold and then deposited on an electrode surface can be reduced at a convenient rate at low voltage (Ag/AgCl) without an electron transfer mediator. Bioelectrodes combining both a colloidal gold-adsorbed oxidase and colloidal gold-adsorbed HRP located on an electrode surface are efficient biodetectors, particularly for the measurement of low glucose levels in samples when glucose oxidase is employed as the sensing enzyme. The biodetectors may be employed for mediatorless detection of a wide variety of analytes depending on the oxidase employed.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.