Immunoassay for detecting group B streptococcus

US5225331A · kind A · utility

Assignees

• National Research Council of Canada · CA
• President and Fellows of Harvard College · US
• The Brigham and Women's Hospital, Inc. · US

Inventors

• Martial Lacroix · Brossard, CA
• Robert Feldman · London, GB
• Dennis L. Kasper · Newton, US
• Harold J. Jennings · Ottawa, CA
• Francis Michon · Laurel, US
• Robert Chalifour · Kirkland, CA
• Vince Pozsgay · Washington, US

Key dates

• Filing date: Apr 25, 1991
• Grant date: Jul 6, 1993
• Expiry date: Apr 25, 2011

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S435/975
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

Immunoadsorbent combinations for the detection and diagnosis of group B streptococcus polysaccharide antigen, comprising an insoluble carrier, a capture agent having an affinity for specifically binding to the trirhamnose epitope of group B streptococcus antigen and having the formula .alpha.-L-Rhap(1.fwdarw.2)-.alpha.-L-Rhap(1.fwdarw.2).alpha.-Rhap- 1- wherein Rhap is rhamnose, and an antigen marker agent having an affinity for binding to monorhamnose epitope of group B streptococcus polysaccharide antigen of formula .alpha.-L-Rhap-1- when the group B streptococcus polysaccharide is bound to the carrier. An immunoassay method test kit and polyclonal antibody are also described.