General method for producing and selecting peptides with specific properties
US5225533A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Mar 22, 1991 |
| Grant date | Jul 6, 1993 |
| Priority date | — |
| Expiry date | Mar 22, 2011 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K1/36
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method to obtain selected individual peptides or families thereof which have a target property and optionally to determine the amino acid sequence of a selected peptide or peptides to permit synthesis in practical quantities is disclosed. In general outline, the method of the invention comprises synthesizing a mixture of randomly or deliberately generated peptides using standard synthesis techniques, but adjusting the individual concentrations of the components of a mixture of sequentially added amino acids according to the coupling constants for each amino acid/amino acid coupling. The subgroup of peptides having the target property can then be selected, and either each peptide isolated and sequenced, or analysis performed on the mixture to permit its composition to be reproduced. Also included in the invention is an efficient method to determine the relevant coupling constants.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.