Patent · US Expired

Catalyzed enzyme electrodes

US5227042A · kind A · utility

276Cited by
3References
12Claims
0Family size

Assignee

Inventors

Key dates

Filing dateMay 15, 1992
Grant dateJul 13, 1993
Priority date
Expiry dateMay 15, 2012

Classification

  • Technology area (CPC Y)Emerging Cross-Sectional Technologies
  • CPC primaryY10S435/817
  • WIPO fieldBiotechnology
  • WIPO sectorChemistry

Abstract

An enzyme electrode is prepared with a composite coating on an electrical conductor. The composite coating is formed from a casting solution of a perfluorosulfonic acid polymer, an enzyme, and a carbon supported catalyst. The solution may be cast directly on the conductor surface or may be formed as a membrane and applied to the surface. The perfluorosulfonic acid ionomer formed from the casting solution provides an insoluble biocompatible protective matrix for the enzyme and acts to retain the enzyme for long term availability in the electrode structure. The carbon supported catalyst provides catalytic sites throughout the layer for the oxidation of hydrogen peroxide from the enzyme reactions. The carbon support then provides a conductive path for establishing an electrical signal to the electrical conductor. In one embodiment, the electrical conductor is a carbon cloth that permits oxygen or other gas to be introduced to the perfluorosulfonic polymer to promote the enzyme reaction independent of oxygen in the solution being tested.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.