Process for isolating A83543 and its components
US5227295A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Nov 8, 1991 |
| Grant date | Jul 13, 1993 |
| Priority date | — |
| Expiry date | Nov 8, 2011 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/822
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
An improved process for isolating A83543 factors from fermentation broth in which they are produced which comprises: PA0 a) adding an approximately equal volume of a water miscible, polar organic solvent to the fermentation broth, including the biomass thereof; PA0 b) separating the liquid phase of the resulting mixture from the biomass; PA0 c) adjusting the pH of the separated liquid phase to between about 7 and 13; PA0 d) applying the separated liquid phase directly to a column of nonfunctional, macroreticular polymer; PA0 e) eluting the A83543 components from the column with an aqueous solution of water miscible, polar organic solvent; and PA0 f) collecting the fractions containing A83543 components.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.