Bradyrhizobium japonicum nodulation inducing factor
US5229113A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Aug 9, 1989 |
| Grant date | Jul 20, 1993 |
| Priority date | — |
| Expiry date | Aug 9, 2009 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/878
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The chemical compounds that induce expression of nodulation genes of Bradyrhizobium japonicum strains have been identified. The isoflavones daidzein and genistein were identified as soybean exudate components that induce nod-genes. Other isoflavones, including 7-hydroxyisoflavone, 5,7-dihydroxyisoflavone, biochanin A, formononetin and prunetin, were also found to act as inducers. In addition, several flavonoid compounds including 4',7-dihydroxyflavone, apigenin, kaempferol and coumestrol, were also found to act as inducers. Nodulation inducing compositions and a method for selectively activating the expression of a gene under the control of a soybean exudate inducible promoter in B. japonicum in response to these inducer molecules are described. In addition, soybean inoculating compositions containing the nod-gene inducers and inoculating methods employing these compositions are described.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.