Patent · US Expired

Preparation of biosensor having a layer containing an enzyme, electron acceptor and hydrophilic polymer on an electrode system

US5229282A · kind A · utility

587Cited by
2References
8Claims
0Family size

Assignee

Inventors

Key dates

Filing dateNov 26, 1990
Grant dateJul 20, 1993
Priority date
Expiry dateNov 26, 2010

Classification

  • Technology area (CPC Y)Emerging Cross-Sectional Technologies
  • CPC primaryY10S435/817
  • WIPO fieldBiotechnology
  • WIPO sectorChemistry

Abstract

The present invention relates to a process of preparation of a biosensor comprising forming an electrode system mainly containing carbon on an insulating base plate, treating the surface of electrode system with an organic solvent, and then arranging a reaction layer on the electrode system to give a unified element. The reaction layer contains an enzyme, electron acceptor and a hydrophilic polymer. Treatment with the organic solvent improves adhesion of the reaction layer to the electrode system. The electrode system contains a working electrode and a counter electrode. The electrode system is formed from a carbon paste containing a resin binder. Treatment is preferably carried out by wiping the surface of the electrodes with a material impregnated by the organic solvent.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.