Thermostable luciferase of firefly, thermostable luciferase gene of firefly, novel recombinant DNA, and process for the preparation of thermostable luciferase of firefly
US5229285A · kind A · utility
65Cited by
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5Claims
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Key dates
| Filing date | Jun 23, 1992 |
| Grant date | Jul 20, 1993 |
| Priority date | — |
| Expiry date | Jun 23, 2012 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y113/12007
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to thermostable luciferase of firefly wherein an amino acid at the 217-position of the amino acid sequence of wild-type firefly luciferase or an amino acid equivalent to the amino acid at the 217-position of luciferase of GENJI firefly or HEIKE firefly is converted into a hydrophobic amino acid, a gene encoding said thermostable luciferase, a vector comprising the gene encoding said thermostable luciferase inserted therein, and a process for the preparation of thermostable firefly luciferase comprising use of said vector.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.