Reagent for use in automatic analyzers for distinguisher leukocyte sub-populations in blood samples
US5232857A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Nov 19, 1990 |
| Grant date | Aug 3, 1993 |
| Priority date | — |
| Expiry date | Nov 19, 2010 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/108331
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention relates to a reagent and to a method of using the same for automatically determining at least one leukocyte sub-population from a total blood sample. Using this reagent, it is possible, in a single step, to lyse the erythrocytes through the action of saponin and SDS, as well as to ensure the differential staining of the leukocyte sub-populations, at the same time preserving the integrity and morphology of the cells, through the combined action of various components including chlorazol black, an alcohol, a surfactant and preserving agents. The reagent according to the invention can be applied to different types of automatic flow cytometry analyzers.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.