Apparatus for direct blotting and automated electrophoresis, transfer and detection and processes utilizing the apparatus thereof
US5234559A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Sep 16, 1992 |
| Grant date | Aug 10, 1993 |
| Priority date | — |
| Expiry date | Sep 16, 2012 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N27/44717
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A separation and direct blot transfer module useful in gel electrophoresis is disclosed in which a transfer membrane stabilized by a frame is moved substantially orthogonally relative to the plane of the separation gel while in minimal contact with the end of the gel toward which molecular fragments are driven electrophoretically. The frame moves at a controlled velocity in a selected direction away from a line of contact that generates a controlled tension in the membrane which is a function of the loading on the line of contact. The module is particularly amenable to automated processing. Apparatus for automated separation, transfer, membrane processing, detection and identification is disclosed in which the module of the invention is employed.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.