Exopeptidase catalyzed site-specific bonding of supports, labels and bioactive agents to proteins

US5234820A · kind A · utility

Assignees

• BOARD OF REGENTS OF THE UNIVERSITY OF NEBRASKA · US
• Bionebraska, Inc. · US

Inventors

• Fred W. Wagner · Walton, US
• Thomas R. Coolidge · Falls Village, US
• Sheldon M. Schuster · Gainesville, US
• Jay Stout · Lincoln, US
• Dwane E. Wylie · Lincoln, US
• Klaus Breddam · Roskilde, DK
• William J. Lewis · Lincoln, US

Key dates

• Filing date: Jun 28, 1990
• Grant date: Aug 10, 1993
• Expiry date: Jun 28, 2010

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S530/816
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

An auxiliary substance such as a label, support, or bioactive agent is attached to a protein at a site that is remote from the active site of the protein by the use of exopeptidase and a nucleophile which is an amino acid, amino acid derivative, amine or alcohol. In one embodiment, the nucleophile is attached to the carboxy terminus of a protein by catalysis with exopeptidase to form an adduct and then the adduct or its combination with a linker arm is bound to the auxiliary substance. In another embodiment, the auxiliary substance or its combination with a linker arm is bound to the nucleophile to form an intermediate substance which is then coupled by catalysis with exopeptidase to the carboxy terminus of a protein.