Device for enhancing fluorescence and kinetics and methods of using the device
US5236827A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Feb 12, 1992 |
| Grant date | Aug 17, 1993 |
| Priority date | — |
| Expiry date | Feb 12, 2012 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S436/80
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A carrier having at least one kinetics and fluorescence enhancing support and a dry substance selected from the group consisting of fluorogenic substrates, B methylumbelliferone, 7-amino-4-methyl coumarin, B-napthylamine, fluoroscein, and resorufin deposited on the support demonstrates substantial enhancement of hydrolysis kinetics and fluorescence over pure liquid systems. When the device has a plurality of supports and the supports have different fluorogenic substrates an enzyme rate-of-reaction profile representative of a microorganism in the suspension can be determined and used to identify the organism. The device can also be used to characterize enzymes expressed by other biological specimens.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.